FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-SS5a_5980_AACT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-SS5a_5980_AACT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51496882
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1177110.22857888755284253Illumina Paired End PCR Primer 2 (100% over 43bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656130.12741159746331826No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT600990.11670415307862717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG146800.033.938773
GACCGAT147100.033.44714736
ACCGATC153200.032.1936137
GATCGGA162000.031.2353485
CGAGACC167600.029.66551633
GATCTCG170550.029.08375740
AATGCCG174550.028.58690328
AGCGGTT177400.028.56645214
AGATCGG179750.028.3622684
AGACCGA176050.028.16258235
CCGAGAC176850.028.14775832
CGGTTCA178650.028.1329416
GAGACCG178600.027.91709934
CCGATCT177050.027.83531438
GAGCGGT182650.027.73440413
CGATCTC180450.027.44374839
GCGGTTC185250.027.34618215
ATCGGAA190050.026.7416
ATGCCGA188150.026.59512329
GTTAGAT191050.026.2775461
Produced by FastQC (version 0.11.2)