FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-PyN5_5989_TGCT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-PyN5_5989_TGCT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15515991
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32900.021.63984912
CAACGCA35250.020.25412213
ATCAACG37200.019.2460511
AACGCAG40850.017.62441814
ACGCAGA45350.015.7880415
GGTATCA46100.015.6172488
GTATCAA46750.015.5284939
CGCAGAG49550.014.65175516
TGGTATC49700.014.52728757
GTGGTAT50250.014.487926
GAGTACT41550.014.00747520
AGAGTAC51300.013.99649119
TATCAAC52450.013.87906510
AGTGGTA56250.012.8358735
TATGCCG7650.011.50388540
CGAGCAA24600.011.46358718
GATCGGA21550.010.95158225
CAGAGTA69050.010.91978718
GTACTTT53450.010.85146122
CCGAGCA27350.010.82276217
Produced by FastQC (version 0.11.2)