FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-PyN4_5981_TTGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-PyN4_5981_TTGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45563903
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG2452900.5383428193146667Illumina Paired End PCR Primer 2 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA284550.036.964725
GACCGAT280700.036.5233836
ACCGATC285150.035.8972937
AAGATCG297450.035.3011743
AATGCCG293850.035.2957228
AGATCGG300800.035.0408174
CGGTTCA298500.035.01492316
AGCGGTT301900.034.7379214
CCGATCT293650.034.7372538
GAGCGGT303250.034.57660313
GATCTCG298300.034.31637240
CGATCTC300900.034.04651339
CGAGACC303600.034.0111533
GAGACCG303700.033.947934
AGACCGA304500.033.83262335
GCGGTTC312950.033.4932915
CCGAGAC310950.033.4385432
AAGAGCG316600.033.23199511
ATCGGAA319300.032.9918986
TGCCGAG315950.032.85858530
Produced by FastQC (version 0.11.2)