FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-MP10_5982_ACGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-MP10_5982_ACGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49045276
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT859910.17532983196995364No Hit
CGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG730310.14890526867460183Illumina Paired End PCR Primer 2 (100% over 43bp)
GTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT516620.10533532322256683Illumina Paired End PCR Primer 2 (100% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT114900.028.54841
TCAACGC158700.027.7736712
CAACGCA163750.027.00290513
ATCAACG178650.024.82908211
AACGCAG185400.023.97916614
ACGCAGA197050.022.48103115
GGTATCA202000.021.9690468
GTATCAA204950.021.740769
CTCGTAT82650.021.65902940
CGCAGAG210650.021.04907216
TAGATCG147950.020.9943733
GTGGTAT216050.020.6069956
TGGTATC218100.020.4497557
GAGTACT183200.020.00034920
AGAGTAC221200.019.86490419
AGTGGTA229950.019.5700935
TATCAAC232100.019.37857210
GTGATCG51550.019.01172
TCTCGTA98750.018.3100439
ATCTCGT186150.017.56764440
Produced by FastQC (version 0.11.2)