FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-EP8a_5986_GAAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-EP8a_5986_GAAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31696741
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT28952729.13428923181724Illumina Paired End PCR Primer 2 (100% over 44bp)
TTCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG3399601.0725392872409185Illumina Paired End PCR Primer 2 (100% over 43bp)
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT1536470.4847406867475744Illumina Paired End PCR Primer 2 (100% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA631320.19917505083566792No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT527110.16629785377619738No Hit
TCAGATCCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG379070.11959273667914312Illumina Paired End PCR Primer 2 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT3284750.038.33208540
TAGATCG170000.038.3079872
GATCGGA3617750.035.5817874
AGATCGG3641350.035.537453
CAGATCG3482550.035.3048062
ATCGGAA3651450.035.2601935
TCGGAAG3691150.034.899386
TCAGATC3587050.034.818481
CGGAAGA3780100.034.130697
AGCGGTT3778150.034.07065213
CGGTTCA3773200.034.04555515
GAGCGGT3785200.034.03170412
AAGAGCG3793300.034.00707610
GCGGTTC3783400.033.99324414
AGAGCGG3797000.033.96724311
AATGCCG3761600.033.92581627
GACCGAT3732150.033.79991535
ATGCCGA3775550.033.7879128
TGCCGAG3773150.033.7766429
ACCGATC3729400.033.7721736
Produced by FastQC (version 0.11.2)