FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-BP5b_5987_TATT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-BP5b_5987_TATT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28278866
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG19420576.867520783895649Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT337740.11943194610420375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318400.11259291656178859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2082000.039.4462365
AAGATCG2092450.039.332063
AGATCGG2097650.039.1873554
AGCGGTT2088550.039.16469214
CGGTTCA2084700.039.1423416
TAAGATC2105550.039.118652
GAGCGGT2093550.039.1016513
ATCGGAA2100550.039.0940746
ATAAGAT2111500.039.0655251
GCGGTTC2094400.039.02332315
AAGAGCG2104900.038.98656511
AGAGCGG2107050.038.92400712
AATGCCG2082350.038.86778328
TCGGAAG2115200.038.8190427
GACCGAT2061900.038.7552536
ACCGATC2063150.038.70940837
GATCTCG2066950.038.6727140
ATGCCGA2094050.038.6534829
CGAGACC2078300.038.63867633
TGCCGAG2093400.038.62952430
Produced by FastQC (version 0.11.2)