FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-BP5a_5984_ATAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-BP5a_5984_ATAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55813171
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT49651878.896084761068316Illumina Paired End PCR Primer 2 (100% over 44bp)
ATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT48896628.760767238973036Illumina Paired End PCR Primer 2 (100% over 44bp)
TATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG9955271.7836775480826919Illumina Paired End PCR Primer 2 (100% over 43bp)
ATAGACGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA2794260.5006452688380669Illumina Paired End PCR Primer 2 (100% over 41bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT2737430.4904630844214173Illumina Paired End PCR Primer 2 (100% over 46bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT612890.10981099783776843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC5328750.039.574091
TAGACGG329100.037.5042882
ATAGACG326200.037.0837331
TATAGAT1166450.036.8318671
ATCTCGT11191600.036.58847840
TAGATCG11609100.036.1446152
AGATCGG11934050.035.1958963
GATCGGA11909300.035.1653064
ATCGGAA11977250.034.962535
TCGGAAG12049200.034.755916
GACGGAA359500.033.958944
CGGAAGA12435450.033.722877
AGCGGTT12441200.033.6257213
GAGCGGT12453350.033.6209812
CGGTTCA12423250.033.6148615
AAGAGCG12475950.033.61438410
AGAGCGG12480350.033.59675211
GCGGTTC12447350.033.57459314
AATGCCG12418350.033.41284627
GGTTCAG12524850.033.34485616
Produced by FastQC (version 0.11.2)