FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-BP10_5983_CAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-BP10_5983_CAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42107876
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG1525210.3622148977545198Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT865430.2055268710300182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA422210.10026865282874871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA193350.035.118335
GACCGAT184900.035.1008436
ACCGATC190400.034.16031337
CGGTTCA198650.034.0897816
AGCGGTT203200.033.36375814
AATGCCG200400.033.35198228
CGAGACC196950.033.18568833
AGATCGG208700.032.6312644
GAGCGGT208250.032.62184513
GATCTCG199150.032.5907440
CCGATCT199600.032.48739638
GAGACCG202700.032.25490634
CGATCTC203000.031.99242639
GAGATCG212150.031.8275173
AGACCGA205650.031.76322735
GCGGTTC213850.031.69394315
CCGAGAC209350.031.40143232
AAGAGCG219450.031.09344511
TGCCGAG215550.031.07325430
AGAGCGG221450.030.81266412
Produced by FastQC (version 0.11.2)