FastQCFastQC Report
Mon 1 Dec 2014
2014-01-16-75k2_5988_GCTT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2014-01-16-75k2_5988_GCTT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30443776
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG24315117.9868903252999885Illumina Paired End PCR Primer 2 (100% over 43bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604040.19841165563693544No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT549370.18045396208407263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2579900.039.6329845
AGATCGG2592050.039.4714
CAGATCG2599600.039.392463
CGGTTCA2588700.039.2412916
AGCGGTT2591950.039.24062714
AAGAGCG2604700.039.1753611
ATCGGAA2608850.039.1625186
AGAGCGG2605850.039.11950712
GAGCGGT2602500.039.10851313
AATGCCG2580650.039.06126828
GCGGTTC2604500.039.04015715
TCGGAAG2617350.039.02177
GACCGAT2557100.038.921636
TGCCGAG2589700.038.88170630
ACCGATC2560600.038.84874737
GATCTCG2564500.038.845240
CGAGACC2578900.038.8307533
GCCGAGA2594200.038.8142631
CCGAGAC2585350.038.8119432
GAGACCG2575950.038.7721534
Produced by FastQC (version 0.11.2)