FastQCFastQC Report
Mon 1 Dec 2014
2013-08-20_2013-09-03-SS10_4936_TTGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2013-08-20_2013-09-03-SS10_4936_TTGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41387937
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1515200.36609701034385933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580120.14016644511660487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC259150.028.00677512
CAACGCA263300.027.60299313
ATCAACG271100.026.84601811
GACCGAT60950.025.66155636
AACGCAG284300.025.54976714
GATCGGA63200.025.409985
GGTATCA289400.025.2791258
GAGTACT262500.024.89299420
GTATCAA293650.024.8726679
GTGGTAT295750.024.75626
ACGCAGA295400.024.57090615
ACCGATC64650.024.50228537
AGTGGTA301100.024.3759295
TGGTATC302700.024.2341337
CGCAGAG309850.023.45767216
TATCAAC313850.023.3930310
AGAGTAC318650.022.65951319
AATGCCG71650.022.2177828
GTACTTT299400.021.97201522
AGCGGTT73700.021.84471114
Produced by FastQC (version 0.11.2)