FastQCFastQC Report
Mon 1 Dec 2014
2013-08-20_2013-09-03-PV10_4935_AACT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2013-08-20_2013-09-03-PV10_4935_AACT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41906949
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1115670.2662255369628555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542860.1295393754386653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC240950.030.8441812
CAACGCA244050.030.50144813
ATCAACG251350.029.62368811
AACGCAG264550.028.20582614
GGTATCA266750.027.9655028
ACGCAGA270650.027.48938815
GTATCAA272600.027.4023069
GTGGTAT274850.027.2721586
TGGTATC282050.026.4408997
CGCAGAG284800.026.1307516
TATCAAC287400.026.12347210
AGTGGTA289900.025.8701655
AGAGTAC287100.025.57346719
GAGTACT199500.024.01057820
CAGAGTA332850.022.20858218
AAGCAGT351100.022.0044781
GTACTTT225750.021.27180922
TAGATCG68100.020.792343
CAGTGGT365150.020.7689344
GCAGAGT363450.020.575217
Produced by FastQC (version 0.11.2)