2013-08-20_2013-09-03-P12P_4938_ATAT.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	2013-08-20_2013-09-03-P12P_4938_ATAT.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34641059
Sequences flagged as poor quality	0
Sequence length	46
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT	5981825	17.26802001058917	Illumina Paired End PCR Primer 2 (100% over 44bp)
TTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGT	5232225	15.104113878273756	Illumina Paired End PCR Primer 2 (100% over 44bp)
ATAGACGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA	466309	1.346116468321595	Illumina Paired End PCR Primer 2 (100% over 41bp)
TATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG	336583	0.9716302264315879	Illumina Paired End PCR Primer 2 (100% over 43bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT	322364	0.9305835598155356	Illumina Paired End PCR Primer 2 (100% over 46bp)
ATAGATGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA	57627	0.16635461404341015	Illumina Paired End PCR Primer 2 (97% over 41bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTAGATC	566680	39.809162	1
TAGACGG	54225	38.993587	2
ATAGACG	54155	38.955532	1
TAGATCG	1244505	38.692226	2
ATAGATC	688075	37.66391	1
AGATCGG	1280770	37.58807	3
GATCGGA	1276045	37.52947	4
ATCGGAA	1281540	37.362785	5
GACGGAA	55700	37.210606	4
TCGGAAG	1290915	37.13377	6
AGACGGA	56405	36.869606	3
ATCTCGT	1316075	36.10131	40
CGGAAGA	1354195	35.502876	7
TATAGAT	41370	35.467407	1
ACGGAAG	58380	35.16701	5
GAGCGGT	1374075	35.129414	12
AAGAGCG	1372645	35.127163	10
AGCGGTT	1370850	35.118217	13
GCGGTTC	1367125	35.105145	14
CGGTTCA	1364975	35.09197	15