FastQCFastQC Report
Mon 1 Dec 2014
2013-08-20_2013-09-03-Ch10_4937_CAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2013-08-20_2013-09-03-Ch10_4937_CAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55024363
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG6807151.2371156391215288Illumina Paired End PCR Primer 2 (100% over 43bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2601320.4727578581872906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1756110.3191513548280423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA789100.037.36385
GACCGAT771400.037.19463736
ACCGATC772900.037.0810537
CGGTTCA798300.036.80302416
GAGCGGT805300.036.75795413
AGCGGTT803400.036.75762614
AGATCGG804700.036.741564
AATGCCG802900.036.64594328
GAGATCG809550.036.565883
GATCTCG787450.036.50104540
CCGATCT784050.036.4968738
CGAGACC795550.036.4160433
GCGGTTC810850.036.3022415
ATCGGAA815500.036.178596
GAGACCG799550.036.1643734
ATGCCGA815900.036.08904629
CGATCTC795800.036.05770539
AGACCGA800600.035.94028535
AAGAGCG826900.035.84876311
AGAGCGG829350.035.80797212
Produced by FastQC (version 0.11.2)